7DUG
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with mRNA and cognate transfer RNA anticodon stem-loop and sisomicin derivative N1''TFMS bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9898 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 400.000, 400.000, 173.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.780 - 3.750 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.22500 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (DEV-3318) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.779 | 2.980 |
| High resolution limit [Å] | 2.910 | 2.920 |
| Rmerge | 0.910 | 9.901 |
| Number of reflections | 287028 | |
| <I/σ(I)> | 6.58 | 0.51 |
| Completeness [%] | 94.5 | 53.8 |
| Redundancy | 84.9 | 36.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 17% MPD |
