7DUF

Crystal structure of VIM1 PHD finger.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1.28266
Spacegroup name	P 31 2 1
Unit cell lengths	74.590, 74.590, 58.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.300 - 2.610
R-factor	0.2157
Rwork	0.215
R-free	0.23170
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.591
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.300	2.700
High resolution limit [Å]	2.610	2.610
Rmerge	0.079	0.662
Number of reflections	11062	599
<I/σ(I)>	25.77	3.53
Completeness [%]	98.9	98.34
Redundancy	10.3
CC(1/2)	0.998	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1M HEPES, 2M Ammonium sulfate