7DU7
Crystal structure of the rationally designed mkDPBB_sym1 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-29 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1 |
Spacegroup name | P 31 |
Unit cell lengths | 31.259, 31.259, 132.177 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.071 - 1.201 |
R-factor | 0.2045 |
Rwork | 0.204 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7du6 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.270 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 44946 | 7142 |
<I/σ(I)> | 16.14 | |
Completeness [%] | 99.7 | |
Redundancy | 4.88 | |
CC(1/2) | 0.999 | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2100mM Maleic acid |