7DU4
The structure of the M.tb MazF-mt9 toxin in complex with a fragment of cognate antitoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 |
| Unit cell lengths | 74.610, 74.610, 104.354 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.310 - 2.180 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a6x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.987 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.200 |
| Rmerge | 0.205 | 0.760 |
| Number of reflections | 17270 | 1730 |
| <I/σ(I)> | 13.2 | 4.15 |
| Completeness [%] | 100.0 | |
| Redundancy | 19.2 | |
| CC(1/2) | 0.935 | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 1.5 M NaCl,0.1 M NaOAc pH 5.0 |
