7DTO
Human Annexin A2 with C132-C261 intramolecular disulfide bond
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | RIGAKU R-AXIS IV |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-03-22 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.050, 49.292, 57.355 |
Unit cell angles | 90.00, 105.36, 90.00 |
Refinement procedure
Resolution | 55.310 - 2.800 |
R-factor | 0.2187 |
Rwork | 0.218 |
R-free | 0.22250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hyu |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.310 | 2.873 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.219 | 0.298 |
Number of reflections | 29312 | 521 |
<I/σ(I)> | 4.215 | |
Completeness [%] | 98.7 | |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 289 | 13% PEG 550 MME, 0.1 M Bis-Tris pH 6.5, 50 mM NaCl |