7DTK
Crystal structure of the RecA1 domain of RNA helicase CGH-1 in C. elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.754, 44.091, 54.213 |
| Unit cell angles | 95.49, 96.32, 100.77 |
Refinement procedure
| Resolution | 35.656 - 1.849 |
| R-factor | 0.1738 |
| Rwork | 0.172 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ct4 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.656 | 1.915 |
| High resolution limit [Å] | 1.849 | 1.849 |
| Rmerge | 0.045 | 0.318 |
| Number of reflections | 31650 | 3059 |
| <I/σ(I)> | 20.404 | 2.95 |
| Completeness [%] | 96.4 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 25% PEG 3350, 0.2M Sodium Chloride, 0.1M Hepes pH 7.5 |
