7DTB

Room tempeature structure of lysozyme by fixed-target serial crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	P 43 21 2
Unit cell lengths	79.450, 79.450, 38.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.180 - 1.650
R-factor	0.1815
Rwork	0.178
R-free	0.20870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cjz
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	15398	1498
<I/σ(I)>	6.58	1.58
Completeness [%]	100.0	99.93
Redundancy	83.7
CC(1/2)	0.984	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293.5	sodium acetate, PEG 8000, NaCl