7DT6
Crystal structure of V30M mutated transthyretin in complex with purpurin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-18 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 42.930, 85.430, 63.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.280 - 1.300 |
R-factor | 0.1882 |
Rwork | 0.187 |
R-free | 0.21120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pwe |
RMSD bond length | 0.006 |
RMSD bond angle | 0.893 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1-2155_1069: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.900 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmeas | 0.076 | 0.994 |
Rpim | 0.025 | 0.492 |
Number of reflections | 56091 | 5685 |
<I/σ(I)> | 15.5 | 1.8 |
Completeness [%] | 96.0 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG400, CaC2l, NaOAc |