7DT1
The structure of Lactobacillus fermentum 4,6-alpha-Glucanotransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-21 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 99.830, 106.080, 106.020 |
Unit cell angles | 90.00, 104.83, 90.00 |
Refinement procedure
Resolution | 48.726 - 2.430 |
R-factor | 0.196616983888 |
Rwork | 0.194 |
R-free | 0.25648 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jbe |
RMSD bond length | 0.007 |
RMSD bond angle | 0.866 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.730 | 2.480 |
High resolution limit [Å] | 2.430 | 2.430 |
Number of reflections | 80425 | 4541 |
<I/σ(I)> | 7.4 | |
Completeness [%] | 99.9 | |
Redundancy | 6.9 | |
CC(1/2) | 0.993 | 0.827 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M imidazole/hydrochloric acid buffer pH 8.5, 0.2 M calcium acetate , 10% (w/v) PEG 8000 |