7DT0

Proline hydroxylase H11-N101I mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	121.910, 138.640, 152.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.600 - 2.430
R-factor	0.2044
Rwork	0.201
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6LNS
RMSD bond length	0.016
RMSD bond angle	1.856
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.600	2.510
High resolution limit [Å]	2.430	2.430
Rmerge	0.127	0.721
Rmeas		0.773
Number of reflections	97664	8710
<I/σ(I)>	35.44
Completeness [%]	99.8
Redundancy	13.4
CC(1/2)	0.998	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M Potassium sodium tartrate tetrahydrate, 16% w/v Polyethylene glycol 3350