7DSZ
Crystal structure of Amuc_1102 from Akkermansia muciniphila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.469, 83.941, 107.252 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.644 - 1.750 |
R-factor | 0.1733 |
Rwork | 0.172 |
R-free | 0.19990 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
Rmerge | 0.074 | 0.030 | 0.822 |
Rmeas | 0.080 | 0.033 | 0.896 |
Rpim | 0.029 | 0.012 | 0.351 |
Total number of observations | 555345 | ||
Number of reflections | 76720 | 8048 | 7572 |
<I/σ(I)> | 5.6 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 7.2 | 7 | 6.3 |
CC(1/2) | 0.999 | 0.686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M Sodium acetate, pH 4.6, and 2 M NaCl |