7DSU

Structure of Mod subunit of the Type III restriction-modification enzyme Mbo45V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-30
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.97887
Spacegroup name	P 21 21 21
Unit cell lengths	95.296, 100.875, 187.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.703 - 3.200
R-factor	0.2288
Rwork	0.226
R-free	0.28530
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.703	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.132	1.102
Number of reflections	60724	4374
<I/σ(I)>	12.8	2.1
Completeness [%]	99.5
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	11% PEG 3350, 0.1 M proline, 0.1 M Na-Hepes pH 7.5