7DSU
Structure of Mod subunit of the Type III restriction-modification enzyme Mbo45V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-30 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 0.97887 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.296, 100.875, 187.316 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.703 - 3.200 |
| R-factor | 0.2288 |
| Rwork | 0.226 |
| R-free | 0.28530 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.652 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.703 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.132 | 1.102 |
| Number of reflections | 60724 | 4374 |
| <I/σ(I)> | 12.8 | 2.1 |
| Completeness [%] | 99.5 | |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 11% PEG 3350, 0.1 M proline, 0.1 M Na-Hepes pH 7.5 |
