7DS8
Crystal structure of actin capping protein in complex with twinflin-1/CD2AP CPI chimera peptide (CDN-TWC)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2020-12-23 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.12 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.690, 63.840, 144.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.530 - 1.950 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ds2 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.731 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.325 | 48.325 | 2.070 |
High resolution limit [Å] | 1.950 | 5.810 | 1.950 |
Rmerge | 0.107 | 0.030 | 0.772 |
Rmeas | 0.116 | 0.033 | 0.832 |
Number of reflections | 41266 | 1697 | 6572 |
<I/σ(I)> | 15.4 | 52.74 | 2.61 |
Completeness [%] | 99.9 | 99 | 99.6 |
Redundancy | 7.165 | 6.461 | 7.108 |
CC(1/2) | 0.998 | 0.999 | 0.775 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4% (w/v) PEG 3350, 50mM Tris-HCl (pH = 7.0) |