7DS4

Crystal structure of actin capping protein in complex with twinflin-1 C-terminus tail F323K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	90
Detector technology	CCD
Collection date	2020-09-04
Detector	ADSC QUANTUM 270
Wavelength(s)	1.12
Spacegroup name	P 21 21 21
Unit cell lengths	52.450, 66.720, 164.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.220 - 1.850
R-factor	0.2118
Rwork	0.209
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ds2
RMSD bond length	0.006
RMSD bond angle	0.843
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.220	44.220	1.960
High resolution limit [Å]	1.850	5.510	1.850
Rmerge	0.062	0.030	0.615
Rmeas	0.068	0.033	0.668
Number of reflections	50056	2061	7944
<I/σ(I)>	17.73	46.96	2.63
Completeness [%]	99.8	98.8	99.5
Redundancy	6.353	5.992	6.505
CC(1/2)	0.999	0.999	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	5% (w/v) PEG 3350, 5% (v/v) ethanol, 50mM Tris-HCl (pH = 7.0)