7DS3
Crystal structure of actin capping protein in complex with twinflin-2 C-terminus tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2020-01-16 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.12 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.510, 67.640, 164.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.170 - 2.090 |
R-factor | 0.2168 |
Rwork | 0.215 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ds2 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.541 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.170 | 49.170 | 2.220 |
High resolution limit [Å] | 2.090 | 6.230 | 2.090 |
Rmerge | 0.058 | 0.022 | 0.693 |
Rmeas | 0.062 | 0.024 | 0.748 |
Number of reflections | 34883 | 1440 | 5526 |
<I/σ(I)> | 22.82 | 70.6 | 2.84 |
Completeness [%] | 99.9 | 98 | 99.8 |
Redundancy | 7.148 | 6.235 | 7.133 |
CC(1/2) | 0.999 | 0.999 | 0.834 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5% (w/v) PEG 3350, 50mM Tris-HCl (pH = 7.0) |