7DRV
Structural basis of SARS-CoV-2-closely-related bat coronavirus RaTG13 to hACE2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.276, 122.875, 110.742 |
Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
Resolution | 48.940 - 3.090 |
R-factor | 0.2272 |
Rwork | 0.226 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lzg |
RMSD bond length | 0.002 |
RMSD bond angle | 0.478 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.940 | 3.210 |
High resolution limit [Å] | 3.090 | 3.100 |
Rmerge | 0.106 | 1.310 |
Number of reflections | 39622 | 3806 |
<I/σ(I)> | 14.3 | |
Completeness [%] | 99.5 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M succinic acid pH 7.0, 0.1 M bicine pH 8.5, 30% v/v polyethylene glycol monomethyl ether 550 |