7DRS
Structure of SspE_40224
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-07 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.082, 138.150, 293.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.140 - 3.420 |
| R-factor | 0.2144 |
| Rwork | 0.212 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jiv |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rpim | 0.074 | |
| Number of reflections | 122839 | 6612 |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 91.4 | |
| Redundancy | 13.3 | |
| CC(1/2) | 0.779 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287.15 | 10% PEG MME 5000,5% Tascimate,pH 7.0,0.1M HEPES pH 7.0 |
