7DRR
Structure of SspE-R100A protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-10 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 110.028, 137.970, 292.354 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.540 - 3.480 |
R-factor | 0.2276 |
Rwork | 0.224 |
R-free | 0.29420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jiv |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.480 | 3.480 |
Number of reflections | 56714 | 2809 |
<I/σ(I)> | 2 | |
Completeness [%] | 99.9 | |
Redundancy | 12.9 | |
CC(1/2) | 0.988 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287.15 | 10% PEG 3350, 4% Tacsimate pH 7.0 |