7DQO

Marsupenaeus japonicus ferritin mutant-D132R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	273.15
Detector technology	PIXEL
Collection date	2020-11-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 4
Unit cell lengths	125.448, 125.448, 176.178
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.982 - 1.701
R-factor	0.2408
Rwork	0.240
R-free	0.27560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a4u
RMSD bond length	0.007
RMSD bond angle	0.817
Data scaling software	HKL-2000
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.982	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	297165
<I/σ(I)>	22.77
Completeness [%]	100.0
Redundancy	6.3
CC(1/2)	0.980	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M Tris-HCl (pH 8.5), 30% v/v 2-Propanol