7DQN
The structure of the Arabidopsis thaliana guanosine deaminase mutant Y185F complexed with guanosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-12-16 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 |
Unit cell lengths | 119.171, 119.171, 39.476 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.780 - 2.600 |
R-factor | 0.1682 |
Rwork | 0.165 |
R-free | 0.22140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dbf |
RMSD bond length | 0.003 |
RMSD bond angle | 0.562 |
Data reduction software | CrysalisPro |
Data scaling software | CrysalisPro |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.780 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.162 | 0.609 |
Number of reflections | 10100 | 1442 |
<I/σ(I)> | 12.1 | 3.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.9 | 7.1 |
CC(1/2) | 0.992 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1.2 M Na-citrate and 0.1 M HEPES (pH 7.5) |