7DQG
Crystal structure of Salmonella phage acetyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100.15 |
Detector technology | PIXEL |
Collection date | 2019-10-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97941 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 68.458, 68.458, 218.964 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.220 - 2.210 |
R-factor | 0.2095 |
Rwork | 0.209 |
R-free | 0.22490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hgz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.647 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.077 | 0.324 |
Rmeas | 0.091 | 0.283 |
Rpim | 0.017 | 0.067 |
Number of reflections | 15793 | 718 |
<I/σ(I)> | 36.7 | |
Completeness [%] | 98.7 | |
Redundancy | 21.1 | |
CC(1/2) | 0.994 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287.15 | 0.4 M sodium malonate pH 6.0, 0.1 M MES monohydrate pH 6.0, 0.5% (w/v) PEG 10000 |