7DPK

The structure of the exchange reaction of the Arabidopsis thaliana guanosine deaminase in complex with 7-deazaguaosine by guanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2020-09-02
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	119.027, 119.027, 38.919
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.650 - 2.150
R-factor	0.1736
Rwork	0.172
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dbf
RMSD bond length	0.003
RMSD bond angle	0.532
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.650	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.104	0.368
Number of reflections	17452	2513
<I/σ(I)>	18	4.7
Completeness [%]	99.9	100
Redundancy	7.9	5.4
CC(1/2)	0.997	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.2 M Na-citrate and 0.1 M HEPES (pH 7.5)