7DPD

Human MCM9 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	78.286, 85.900, 111.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.110 - 2.550
R-factor	0.2128
Rwork	0.208
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Rossetta predicted model
RMSD bond length	0.003
RMSD bond angle	0.498
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (dev_3139)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.550	2.550
Rmeas	0.183	0.821
Rpim	0.075	0.382
Number of reflections	25165	1250
<I/σ(I)>	9.62	2.22
Completeness [%]	99.9	99.9
Redundancy	5.6	4.4
CC(1/2)	0.987	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1M Li2SO4, 0.1M ADA pH 6.2-6.5, 13-16% (w/v) PEG 4000