7DP3

Human MCM8 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97930
Spacegroup name	P 1 21 1
Unit cell lengths	46.017, 52.028, 137.221
Unit cell angles	90.00, 91.86, 90.00

Refinement procedure

Resolution	48.650 - 2.550
R-factor	0.2194
Rwork	0.214
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pof
RMSD bond length	0.002
RMSD bond angle	0.556
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.550	2.550
Rpim	0.060	0.333
Number of reflections	21244	1009
<I/σ(I)>	10.79	2
Completeness [%]	98.4	94.2
Redundancy	5.7	4.8
CC(1/2)	0.966	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M (NH4)2SO4, 20.5-22% (w/v) PEG 3350, 0.1M Tris-Cl, pH 8.5