7DOY

The structure of the Arabidopsis thaliana guanosine deaminase in complex with 6-O-methylguanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 61
Unit cell lengths	119.130, 119.130, 39.733
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.610 - 2.170
R-factor	0.175
Rwork	0.173
R-free	0.21210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dbf
RMSD bond length	0.003
RMSD bond angle	0.559
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.170	2.170
Rmerge	0.177	0.501
Rmeas	0.180
Number of reflections	17386	1711
<I/σ(I)>	25.6	6.333
Completeness [%]	100.0	100
Redundancy	19.8	17.7
CC(1/2)	0.992	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.2 M Na-citrate and 0.1 M HEPES (pH 7.5)