7DOX
The structure of the Arabidopsis thaliana guanosine deaminase in complex with m7G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-09-02 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 119.136, 119.136, 39.812 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.850 - 1.900 |
| R-factor | 0.1741 |
| Rwork | 0.173 |
| R-free | 0.19310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dbf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.019 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.850 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.200 | 1.057 |
| Number of reflections | 25788 | 3733 |
| <I/σ(I)> | 10.2 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | |
| CC(1/2) | 0.992 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2 M Na-citrate and 0.1 M HEPES (pH 7.5) |
