7DOW

The structure of the Arabidopsis thaliana guanosine deaminase in reaction with 7-deazaguansoine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-18
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	119.075, 119.075, 39.692
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.660 - 2.000
R-factor	0.1703
Rwork	0.169
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dbf
RMSD bond length	0.003
RMSD bond angle	0.579
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.660	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.073	0.410
Number of reflections	21954	3095
<I/σ(I)>	22.5	4.4
Completeness [%]	98.6	94.8
Redundancy	7.9	4.9
CC(1/2)	0.998	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.2 M Na-citrate and 0.1 M HEPES (pH 7.5)