7DOV
The crystal structure of zebrafish tumor necrosis factor alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.328, 91.021, 63.396 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.270 - 2.590 |
| R-factor | 0.2217 |
| Rwork | 0.218 |
| R-free | 0.29910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tsv |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.633 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.0.077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.310 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.600 |
| Rmerge | 0.096 | 0.852 |
| Number of reflections | 15728 | 1428 |
| <I/σ(I)> | 32.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 25.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277.15 | 0.1 M DL-Malic acid pH 7.0, 12% w/v Polyethylene glycol 3350 |
