7DON
Structure of tubulin H392D mutant from Odinarchaeota
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.210, 92.614, 102.143 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.310 - 1.800 |
Rwork | 0.171 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6o2r |
RMSD bond length | 0.014 |
RMSD bond angle | 1.286 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.310 | 1.870 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.072 | 1.120 |
Rpim | 0.031 | 0.464 |
Number of reflections | 35951 | 3485 |
<I/σ(I)> | 14.2 | 1.6 |
Completeness [%] | 99.7 | |
Redundancy | 6.5 | |
CC(1/2) | 0.999 | 0.678 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M sodium acetate trihydrate, 0.1 M Bis-Tris propane, pH 8.5, 25 % w/v PEG 3350 |