7DOG

Crystal structure of a nuclease and capping domain of SbcD from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-12-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	73.906, 89.465, 116.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.840 - 2.910
R-factor	0.1946
Rwork	0.192
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s6v
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.148	0.333
Rmeas	0.143	0.297
Rpim	0.051	0.138
Number of reflections	16408	749
<I/σ(I)>	10.7	3.5
Completeness [%]	93.8	86.7
Redundancy	5.5	3.5
CC(1/2)	0.989	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	2% (v/v) TacsimateTM (pH 6.0), 0.1 M Bis-Tris (pH 7.0), 18% (w/v) polyethylene glycol 3350