7DO6
Crystal structure of Azotobacter vinelandii L-rhamnose 1-dehydrogenase(NADP bound-form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 78.633, 81.571, 86.389 |
Unit cell angles | 87.68, 75.53, 89.35 |
Refinement procedure
Resolution | 50.030 - 2.370 |
R-factor | 0.2076 |
Rwork | 0.205 |
R-free | 0.25730 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.030 | 50.030 | 2.510 |
High resolution limit [Å] | 2.370 | 7.050 | 2.370 |
Rmerge | 0.205 | 0.071 | 1.003 |
Rmeas | 0.268 | 0.092 | 1.311 |
Total number of observations | 354663 | ||
Number of reflections | 82779 | 6116 | 25333 |
<I/σ(I)> | 3.68 | 11.5 | 0.92 |
Completeness [%] | 96.1 | 94.9 | 93 |
Redundancy | 2.185 | 2.283 | 2.149 |
CC(1/2) | 0.965 | 0.982 | 0.433 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Tris-HCl (pH 8.0), 0.2 M NaCl, 24.5% (w/v) PEG 3350 |