7DO5

Crystal structure of Azotobacter vinelandii L-rhamnose 1-dehydrogenase(apo-form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	75.371, 81.579, 91.180
Unit cell angles	80.45, 74.55, 75.20

Refinement procedure

Resolution	47.587 - 1.836
R-factor	0.1618
Rwork	0.160
R-free	0.18810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2uvd
Data scaling software	XDS
Phasing software	BALBES
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	47.530	2.010
High resolution limit [Å]	1.836	5.650	1.900
Rmerge	0.075	0.035	0.544
Rmeas	0.106	0.049	0.769
Number of reflections	169510	11269	47442
<I/σ(I)>	6.45	17.94	1.18
Completeness [%]	92.6	93.4	92.4
Redundancy	1.842	1.877	1.807
CC(1/2)	0.993	0.995	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	100 mM Tris-HCl (pH 8.5), 200 mM lithium sulfate, 25% (w/v) PEG 3350