7DO4

Crystal structure of CD97-CD55 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	51.800, 44.250, 116.530
Unit cell angles	90.00, 98.70, 90.00

Refinement procedure

Resolution	33.226 - 3.200
R-factor	0.2708
Rwork	0.267
R-free	0.30440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bou 1ojv
RMSD bond length	0.004
RMSD bond angle	0.899
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.230	3.270
High resolution limit [Å]	3.190	3.190
Rmerge	0.140	0.765
Number of reflections	8903	641
<I/σ(I)>	7.3	2.2
Completeness [%]	99.2	98.3
Redundancy	3.8	4
CC(1/2)	0.980	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M MES pH 5.9 and 10% PEG20000