7DNT
mRNA-decapping enzyme g5Rp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2019-07-11 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 56.083, 105.113, 49.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.715 - 2.500 |
R-factor | 0.2029 |
Rwork | 0.194 |
R-free | 0.28510 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.499 | 6.780 | 2.500 |
Rmerge | 0.127 | 0.076 | 0.305 |
Rmeas | 0.134 | 0.081 | 0.346 |
Rpim | 0.041 | 0.025 | 0.157 |
Number of reflections | 17165 | 603 | 248 |
<I/σ(I)> | 7.9 | ||
Completeness [%] | 91.7 | 99.2 | 48.8 |
Redundancy | 9.1 | 9.5 | 3.4 |
CC(1/2) | 0.997 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 289.15 | 0.1M sodium citrate tribasic dihydrate (pH 5.8), 0.54M magnesium formate dihydrate, 10% (v/v) 1,2-butanediol as an additive reagent |