7DNO
Characterization of Peptide Ligands Against WDR5 Isolated Using Phage Display Technique
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.735, 53.912, 64.788 |
| Unit cell angles | 69.82, 88.79, 74.16 |
Refinement procedure
| Resolution | 30.310 - 2.030 |
| R-factor | 0.1817 |
| Rwork | 0.179 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uvl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.056 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.310 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.072 | |
| Number of reflections | 32798 | 3122 |
| <I/σ(I)> | 12.16 | |
| Completeness [%] | 89.0 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.998 | 0.846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M BisTris pH 6.0, 0.2M ammonium acetate, 20-30% PEG3350 |
