7DNA
Photocleavable Fluorescent Protein in green and red form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 |
Unit cell lengths | 38.930, 72.490, 126.550 |
Unit cell angles | 92.46, 97.31, 92.50 |
Refinement procedure
Resolution | 46.270 - 2.300 |
R-factor | 0.2232 |
Rwork | 0.221 |
R-free | 0.27070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hqk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.863 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.270 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.042 | |
Number of reflections | 58440 | 9388 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 95.9 | |
Redundancy | 1.8 | |
CC(1/2) | 0.998 | 0.855 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MIB buffer pH 6.0, 25% w/v PEG 1500 |