7DN9
Crystal structure of Salmonella effector in complex with NAD and host co-factor ARF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-11-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.711, 105.576, 105.839 |
| Unit cell angles | 90.00, 104.80, 90.00 |
Refinement procedure
| Resolution | 46.910 - 3.290 |
| R-factor | 0.2388 |
| Rwork | 0.236 |
| R-free | 0.27680 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.427 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.920 | 3.370 |
| High resolution limit [Å] | 3.287 | 3.290 |
| Rmerge | 0.156 | 0.501 |
| Rmeas | 0.193 | 0.625 |
| Number of reflections | 33860 | 2301 |
| <I/σ(I)> | 5.79 | 2.03 |
| Completeness [%] | 97.1 | 92.6 |
| Redundancy | 2.86 | 2.63 |
| CC(1/2) | 0.975 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 13% PEG 3350, 300mM sodium citrate, 100mM Bis-Tris pH 6.5 |
