7DN8

Crystal structure of Salmonella effector SopF in complex with ARF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97861
Spacegroup name	P 1 21 1
Unit cell lengths	104.737, 104.902, 105.603
Unit cell angles	90.00, 104.88, 90.00

Refinement procedure

Resolution	46.651 - 2.608
R-factor	0.2022
Rwork	0.201
R-free	0.23990
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.486
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.660	2.680
High resolution limit [Å]	2.608	2.608
Rmerge	0.076	0.472
Rmeas	0.091	0.565
Number of reflections	67310	4866
<I/σ(I)>	11.67	2.27
Completeness [%]	99.6	99.6
Redundancy	3.5	3.2
CC(1/2)	0.996	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	10.5% PEG 3350, 120mM alpha-ketoglutarate, 100mM Tris-HCl pH 8.0