7DN1

Hetero-oligomers of SCR-SCR2 crystal structure with NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	69.589, 114.676, 127.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.190 - 1.740
R-factor	0.1677
Rwork	0.166
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ctm
RMSD bond length	0.012
RMSD bond angle	2.094
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.190	1.760
High resolution limit [Å]	1.740	1.740
Number of reflections	100531	3906
<I/σ(I)>	11
Completeness [%]	98.5
Redundancy	10.9
CC(1/2)	0.990	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	1 M MES, 30% PEG 300, pH 6.5