7DMM
Structure of a glucose isomerase crystal grown in an aqueous glycerol solution without any precipitants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2019-06-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 92.610, 98.460, 102.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.160 - 0.990 |
R-factor | 0.15736 |
Rwork | 0.157 |
R-free | 0.16564 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xib |
RMSD bond length | 0.029 |
RMSD bond angle | 2.290 |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.940 | 1.010 |
High resolution limit [Å] | 0.990 | 0.990 |
Rmerge | 0.046 | 0.308 |
Number of reflections | 256074 | 12629 |
<I/σ(I)> | 16.9 | |
Completeness [%] | 99.8 | |
Redundancy | 6.4 | |
CC(1/2) | 0.998 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | precipitant-free in 30(v/v)% aqueous glycerol solution |