7DMA

Crystal structure of FliM middle domain (46-231) with R49P substitution from Vibro alginolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	29.460, 62.990, 75.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.460 - 1.440
R-factor	0.1748
Rwork	0.173
R-free	0.20180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x0z
RMSD bond length	0.008
RMSD bond angle	1.085
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.870	1.470
High resolution limit [Å]	1.440	1.440
Rmerge	0.078	0.481
Rmeas	0.093	0.574
Rpim	0.049	0.307
Number of reflections	26243	1332
<I/σ(I)>	10.5	2.6
Completeness [%]	100.0	100
Redundancy	6.1	5.6
CC(1/2)	0.996	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	30 % (w/v) PEG-400, 0.1 M HEPES-NaOH, 0.2 M NaCl