7DM9

Crystal structure of FliM middle domain (51-229) from Vibro alginolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	29.580, 62.500, 73.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.720 - 1.710
R-factor	0.1748
Rwork	0.173
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x0z
RMSD bond length	0.009
RMSD bond angle	1.177
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.890	1.740
High resolution limit [Å]	1.710	1.710
Rmerge	0.066	0.264
Rmeas	0.079	0.319
Rpim	0.044	0.176
Number of reflections	15496	824
<I/σ(I)>	14.3	5.7
Completeness [%]	100.0	100
Redundancy	5.9	6
CC(1/2)	0.996	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	40 % (w/v) PEG-600, 0.1 M CHES-NaOH