7DM6
The structure of the Arabidopsis thaliana guanosine deaminase complexed with crotonoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-11-18 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 118.794, 118.794, 39.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.770 - 2.050 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dbf |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.535 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.770 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.114 | 0.380 |
| Number of reflections | 20380 | 2966 |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.4 | 9.7 |
| CC(1/2) | 0.998 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2M Na-citrate and 0.1M HEPES (pH 7.5) |
