7DLX
crystal structure of H2AM4>Z-H2B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.582, 73.237, 109.682 |
| Unit cell angles | 90.00, 95.48, 90.00 |
Refinement procedure
| Resolution | 43.769 - 2.395 |
| R-factor | 0.2292 |
| Rwork | 0.227 |
| R-free | 0.27910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wnn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.504 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.073 | |
| Rmeas | 0.073 | |
| Number of reflections | 48153 | 4899 |
| <I/σ(I)> | 22.8 | |
| Completeness [%] | 98.8 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 0.1M BIS-TRIS propane pH 7.0, 4M Sodium nitrate |
