7DLW
Crystal structure of Arabidopsis ACS7 in complex with PPG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 66.877, 69.353, 85.400 |
Unit cell angles | 97.70, 94.97, 106.50 |
Refinement procedure
Resolution | 45.200 - 2.190 |
R-factor | 0.1945 |
Rwork | 0.193 |
R-free | 0.24080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3piu |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.260 |
High resolution limit [Å] | 2.190 | 2.200 |
Rmerge | 0.085 | 0.474 |
Number of reflections | 71190 | 5851 |
<I/σ(I)> | 10.5 | |
Completeness [%] | 96.2 | |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M Tris pH 8.6, 23% (w/v) PEG 3350 |