7DLC
The structure of the Arabidopsis thaliana guanosine deaminase in reaction with N1-methylguanosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-11-26 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 |
Unit cell lengths | 120.102, 120.102, 40.087 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.030 - 2.450 |
R-factor | 0.1721 |
Rwork | 0.170 |
R-free | 0.21710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dbf |
RMSD bond length | 0.006 |
RMSD bond angle | 0.855 |
Data reduction software | CrysalisPro |
Data scaling software | CrysalisPro |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.030 | 2.580 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.240 | 0.943 |
Number of reflections | 12421 | 1793 |
<I/σ(I)> | 8.1 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.3 | 8.6 |
CC(1/2) | 0.988 | 0.742 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2 M Na-citrate and 0.1 M HEPES (pH 7.5) |