7DLC

The structure of the Arabidopsis thaliana guanosine deaminase in reaction with N1-methylguanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-26
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	120.102, 120.102, 40.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.030 - 2.450
R-factor	0.1721
Rwork	0.170
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dbf
RMSD bond length	0.006
RMSD bond angle	0.855
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.030	2.580
High resolution limit [Å]	2.450	2.450
Rmerge	0.240	0.943
Number of reflections	12421	1793
<I/σ(I)>	8.1	2.3
Completeness [%]	99.9	100
Redundancy	8.3	8.6
CC(1/2)	0.988	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.2 M Na-citrate and 0.1 M HEPES (pH 7.5)