7DL1
The mutant E310G/G323S structure of 3,5-DAHDHcca complex with NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97930 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.241, 127.874, 116.994 |
Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
Resolution | 26.736 - 2.720 |
R-factor | 0.194548456194 |
Rwork | 0.192 |
R-free | 0.23878 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.939 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.770 |
High resolution limit [Å] | 2.715 | 2.720 |
Rmerge | 0.165 | 1.121 |
Number of reflections | 41956 | 2097 |
<I/σ(I)> | 14.182 | |
Completeness [%] | 100.0 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 1.0 M LiCl, 0.1 M Tris-HCl buffer (pH 7.0), 11% w/v PEG 6000 |