7DKZ
Structure of plant photosystem I-light harvesting complex I supercomplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2018-07-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 225.652, 168.952, 185.224 |
Unit cell angles | 90.00, 121.39, 90.00 |
Refinement procedure
Resolution | 44.405 - 2.393 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xk8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.676 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.410 | 2.480 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmeas | 0.110 | |
Rpim | 0.040 | |
Number of reflections | 230250 | 21565 |
<I/σ(I)> | 17.8 | |
Completeness [%] | 98.8 | 92.9 |
Redundancy | 6.8 | 6.2 |
CC(1/2) | 0.998 | 0.708 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 285 | sodium chlotide, magnesium chloride, PEG 400 |