7DKZ

Structure of plant photosystem I-light harvesting complex I supercomplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	80
Detector technology	CCD
Collection date	2018-07-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	225.652, 168.952, 185.224
Unit cell angles	90.00, 121.39, 90.00

Refinement procedure

Resolution	44.405 - 2.393
R-factor	0.1909
Rwork	0.189
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xk8
RMSD bond length	0.008
RMSD bond angle	1.676
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.410	2.480
High resolution limit [Å]	2.390	2.390
Rmeas	0.110
Rpim	0.040
Number of reflections	230250	21565
<I/σ(I)>	17.8
Completeness [%]	98.8	92.9
Redundancy	6.8	6.2
CC(1/2)	0.998	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	285	sodium chlotide, magnesium chloride, PEG 400