7DKO
De novo design protein AM2M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-23 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5406 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 61.260, 58.620, 108.348 |
| Unit cell angles | 90.00, 91.34, 90.00 |
Refinement procedure
| Resolution | 12.958 - 2.600 |
| R-factor | 0.2328 |
| Rwork | 0.230 |
| R-free | 0.28920 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.027 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.960 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.123 | 0.659 |
| Number of reflections | 11773 | 549 |
| <I/σ(I)> | 12.42 | 1.15 |
| Completeness [%] | 98.2 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 3.5M Sodium formate, 0.1M Sodium acetate trihydrate pH 4.6 |
