7DKH
Crystal structure of the Ctr9/Paf1/Cdc73/Rtf1 quaternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 97 |
| Detector technology | PIXEL |
| Collection date | 2018-11-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.977910 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 190.195, 190.195, 216.423 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.960 - 2.900 |
| R-factor | 0.1954 |
| Rwork | 0.193 |
| R-free | 0.24780 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.936 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.960 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.173 | 1.756 |
| Rmeas | 0.178 | 1.815 |
| Rpim | 0.044 | 0.455 |
| Number of reflections | 100181 | 9841 |
| <I/σ(I)> | 17 | 2.5 |
| Completeness [%] | 99.9 | |
| Redundancy | 31.2 | |
| CC(1/2) | 0.999 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 8% tascimate, pH 6.0, 12% PEG 3350 |
